Self-indicating and improved resolution analyses e

专利名称：Self-indicating and improved resolution

analyses employing stoichiometric chemicalsubtraction

发明人：Blatt, Joel M.,Charlton, Steven C.,Walter,

Bert,Warchal, Mary Ellen

申请号：EP101762.6申请日：190202公开号：EP0327952B1公开日：19920415

摘要：An analytical method and test composition for determining whether an analyteis present in a test sample at a predetermined concentration level by measuring orobserving a preselected level of indicator response, e.g., color generation. A subtractivereaction is carried out on the test sample prior to the indicator reaction in order tostoichiometrically and specifically consume a predetermined amount of analyte. Theresulting indicator reaction generates the preselected level of indicator response only ifthe analyte is present at the predetermined concentration level or greater. Thesubtractive reaction is enzymatically specific for the analyte in the reaction mixture andthe amount of analyte consumed can be closely controlled by the amount of a regulatingcoreactant present at initiation. The method enables the determination of analytewithout the need for standards or comparators, e.g., color charts, and therefore is self-indicating. In another embodiment, there is provided a method and test composition forimproving an otherwise suboptimal assay response due to oversaturation of theresponse, e.g., color, generated by an indicator reaction.

申请人：MILES INC.

地址：1127 MYRTLE STREET; ELKHART,IN 46515

代理机构：Adrian, Albert, Dr.

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